Genetically modified yeasts in studies of human amyloidosis
نویسندگان
چکیده
Amyloid protein aggregation is a key factor in the development of variety serious diseases humans, commonly named as amyloidoses (Alzheimers and Parkinsons diseases, type II diabetes, etc.), determinant protein-based inheritance lower eukaryotes. In yeast, translation termination Sup35 one most extensively studied amyloidogenic proteins. Aggregation (induction [PSI+] prion) decreases its functional activity leads to suppression nonsense-mutation stop-codons become recognized meaningful more frequently. This phenomenon basis phenotypic detection yeast strains possessing nonsense mutation ade1-14 ADE1 gene.
 Yeast convenient model for genetic, biochemical molecular biology studies. genome can be easily edited plasmids used induction gene expression. suitable analysis mammalian genes proteins thus applied properties associated with human diseases. Phenotyping prion modified amyloid yeast.
 We use genetically yeasts Saccharomyces cerevisiae adopted research. The mutations leading auxotrophy toward certain amino acids (leucine, lysine, tryptophane, histidine) nucleobases (adenine, uracil) were implemented into allowing phenotyping usage investigation Application yeast-based experimental system studies different aspects discussed.
 study was supported by Saint Petersburg State University (project 93025998).
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ژورنال
عنوان ژورنال: Ecological genetics
سال: 2022
ISSN: ['1811-0932', '2411-9202']
DOI: https://doi.org/10.17816/ecogen112321